fibroblast growth medium  (PromoCell)

Journal: bioRxiv

Article Title: Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

doi: 10.64898/2026.01.28.702392

Figure Lengend Snippet: Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.

Article Snippet: Following WNT activation and inhibition phases, cells were replated and matured in fibroblast growth medium (FGM, PromoCell #C-23130) supplemented with Panexin (replacing fetal calf serum), 10 ng/ml FGF2 and 10 μM SB431542, where indicated.

Techniques: Functional Assay, Inhibition

Journal: Advanced NanoBiomed Research

Article Title: Bioprinting‐Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling

doi: 10.1002/anbr.202400148

Figure Lengend Snippet: Figure 1. Development of vascularized EHT module. a) Schematic diagram illustrating the EHT modules and their cell compositions (CM, cardiomyo- cyte; cFB, cardiac fibroblast; EC, endothelial cell). b) Fluorescence images showing harmonious integration of encapsulated cells within tissue as time progressed (green, DiO-labeled CMs and cFBs; red, Red fluorescent protein-tagged HUVECs; scale bar, 500 μm). c) LIVE/DEAD assessment showing cell viability within the EHT modules at days 1 and 14 after tissue fabrication (green, Calcein-AM; red, ethidium homodimer-1; scale bar, 500 μm), and the quantification of signal-positive areas indicating the cell viability (n = 4, biological replicates).

Article Snippet: HCFs were cultured in fibroblast growth medium kit (C-23130, Promocell) supplemented with 1% P/S.

Techniques: Fluorescence, Labeling

Journal: Advanced NanoBiomed Research

Article Title: Bioprinting‐Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling

doi: 10.1002/anbr.202400148

Figure Lengend Snippet: Figure 2. Vascular network formation leading to complex tissue formation and signaling pathway activation. a) IF image exhibiting the structure and distribution of each cell using cardiomyocyte-, fibroblast- and endothelial cell-specific markers within the tissue (green, α-sarcomeric actin; blue, VIM; red, CD31; scale bar, 100 μm). b) RNA sequencing heatmap showing 352 DEGs between CMF and CMFE. c) Bubble plot of KEGG pathway enrichment analysis of DEGs. The gene ratio indicates the ratio of enriched DEGs to background genes.

Article Snippet: HCFs were cultured in fibroblast growth medium kit (C-23130, Promocell) supplemented with 1% P/S.

Techniques: Activation Assay, RNA Sequencing